The Sisters of Saint Francis of Rochester, Minnesota is a Roman Catholic religious congregation for women. The congregation was founded in 1877 by Mother Mary Alfred Moes in the Roman Catholic Diocese of Winona. The motherhouse, which is in Rochester, Minnesota, is called Assisi Heights.
The congregation founded Saint Marys Hospital (Rochester), which is now part of the Mayo Clinic, and it still maintains a close connection with the hospital. The Sisters operate schools and a clinic in Bogotá, Colombia. They also run a spiritual retreat center in Janesville, Minnesota.